Part:BBa_K422012

Pif3 (AarI C-part)

Biological Background

In plants and some bacteria, members of the phytochrome family (photoreceptors) regulate phototaxis, photosynthesis and production of protective pigments in response to light stimuli. The chromophore is encoded in the N-terminal domain of the protein. There are two mayor types of phytochromes, type I (PhyA) and type II (PhyB and PhyC). Both exist in a biologically inactive form Pr absorbing red light and an active configuration Pfr which absorbs far-red light, using a covalently attached tetraphyrrole chromophore for light absorption. Light-switchable gene systems often are based on the phytochrome interacting factor Pif3, a basic protein with a helix-loop-helix motif, which switches on light-induced gene expression. The N-Terminus of Pif3 selectively binds the Prf form of the phytochrome and rapidly dissociates in response to reconversion to the Pr state.

Cloning strategy

BBF RFC28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI. Pif3-C is a C-part compatible for C-Terminal fusion. For more information see [http://2010.igem.org/Team:ETHZ_Basel/Biology/Cloning].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 329
  
• 1000
  COMPATIBLE WITH RFC[1000]